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Microscopy and spectroscopy stage
-190°C - 400°C with liquid nitrogen cooling option
Ø26 mm sample area
Integrated XY sample positioning
Sealed chamber with vacuum capability
Instec's HCS421VXY vacuum-tight stage is designed for applications where a wide temperature range is needed and control over the atmosphere is critical. This stage features a silver heating and cooling block, providing the user with exceptional temperature uniformity. The vacuum-tight configuration can be both gas purged and evacuated to low pressures to protect sensitive samples from moisture and oxygen as well as to study vacuum processes such as freeze drying.
Temperature Control |
mK2000 with programmable precision switching PID method |
Thermal Block |
Silver |
Sample Thermal Cover |
Removable Inner sample cover with additional window |
Minimum Temperature |
-190°C (with optional liquid N2 cooling) |
Maximum Temperature |
400°C |
Temperature Sensor |
100 Ω Platinum RTD |
Maximum Heating Rate |
+100°C per minute at 100°C |
Maximum Cooling Rate |
-50°C per minute at 100°C |
Minimum Heating and Cooling Rate |
±0.01°C per minute |
Temperature Resolution |
0.01°C |
Temperature Stability |
±0.05°C (>25°C), ±0.1°C (<25°C) |
Power supply |
Universal power input |
Software |
Windows software to record and export temperature vs time data |
Optical access |
Reflection and transmission capability |
Optical windows |
Removable and exchangeable windows permit full-spectrum transparency |
Minimum Objective Working Distance |
5 mm |
Minimum Condenser Working Distance |
11.5 mm |
Top Viewing Aperture |
Ø18 mm (Ø22mm glass) |
Top Viewing Angle |
±57.0° from normal |
Transmission Aperture |
Ø2 mm (Ø4mm option available with HCS421VXY+ model) |
Bottom Viewing Aperture |
Ø18 mm (Ø22mm glass) |
Bottom Viewing Angle |
±19.0° from normal |
Window Defrost | External Window Defrost |
Sample Area |
Ø26* mm (XY positioner sample area 15mm x 15mm) *Ø28 mm on previous model (V12.02.01) |
Chamber Height |
2.5 mm |
Sample Loading |
Sample loading requires lid removal |
Sample Positioning |
10 mm fine travel with Vernier XY dials for remote manipulation in sealed chamber |
Atmosphere Control |
Sealed chamber with vacuum capability to control humidity, condensation, and oxidation Includes 1x stainless steel vacuum bellows + 1x machinable aluminum blank by default. Secondary vacuum bellows available by request. |
Frame Cooling |
Integrated frame cooling with optional chiller system |
Mounting |
Base model includes tapped holes on frame and removable side-mounted L-Brackets. Horizontal and vertical mounting adaptors available for select instruments, or by custom design |
Frame Dimensions |
150mm x 86 mm x 22 mm |
Weight |
1350 g |
HCS421VXY Base-Model Options
Add-on options for the HCS421VXY base model are listed below. These items can be added at any time, and can even be installed by the end-user in the field. When adding a cooling system to an existing HCS421VXY system, it may be necessary to upload a new parameter file to the temperature controller.
Active Sample Cooling The standard HCS421VXY supports precision temperature control from room temperature to 400°C. Enable active sample cooling with one of our LN2 suction-pump cooling systems to unlock active cooling rates as high as -50°C/min and a lower temperature limit of -190°C. Note: When cooling below -60, it is highly recommended to utilize the vacuum-tight stage body to prevent condensation and ice formation | |
- Add LN2-P4D3 Cooling System 4-stage LN2 suction pump cooling system with 3L LN2 Dewar PN: LN2-P4D3 |
- Add LN2-P8D10 Cooling System 8-stage LN2 suction pump cooling system with high-capacity 10L LN2 Dewar PN: LN2-P8D3 |
Window Size and Viewing Angle The standard HCS421VXY has a vacuum-tight chamber lid with a viewing aperture of 18mm (22mm glass window) with a viewing angle of ±57.0° from normal. For applications which require a larger observation area and/or a larger viewing angle, alternate chamber lids are available. Larger window sizes increase the viewing area and angle, but comes at the cost of slightly reduced temperature uniformity. Customization of the bottom viewing aperture is possible, but requires upgrading to the HCS421VXY+ model. If large observation windows are required on both the top and bottom surfaces, take a look at Instec's -IRM stage models. | |
- 18mm aperture lid (Standard) PN: R0220-HCS421VXY |
- 27mm aperture lid PN: R03170-HCS421VXY |
Window Material
The HCS421VXY utilizes removable and exchangeable windows to optimize performance for any wavelength. Base-model options are shown below, but specialized options are available per request with the HCS421VXY+ model. Note: The exact window part number depends on desired chamber lid, as multiple sizes are available | |
- Quartz Glass (Standard) PN: WQ1 |
- BaF2 Glass for IR PN: WBaF2 |
- ZnSe Glass for IR PN: WZnSe |
- CaF2 Glass for IR PN: WCaF2 |
- Saphire Glass for UV/IR PN: WSP |
|
Sample Mounting The HCS421VXY is typically used in a horizontal configuration with the integrated XY positioners and uses gravity to secure the sample against the thermal block. In cases where more thermal contact is required, samples are not perfectly flat, or a special mounting solution is desirable, alternate sample mounting options are available for the HCS421VXY. Threaded holes on the sample area are included with each HCS421VXY base model, and a set of up to 3 spring loaded sample clips can be added to conveniently secure tricky samples, or to secure sample when the stage is used in a vertical configuration. Note: Spring-loaded sample clips are NOT compatible with the Integrated XY positioners when used with certain samples (ie. very thin microscope slide covers). Consult sales@instec.com for more information on whether your sample may be used in a vertical configuration. | |
- Threaded holes on thermal block (Standard) Hole dimensions available by request PN: HCS421VXY |
- Add up to 3X sample clips Spring loaded stainless steel sample clips PN: SFC-HCS421VXY |
Environmental Control With its vacuum-tight chamber, the HCS421VXY base model is capable of positive pressure gas-purge up to 0.5 Bar or evacuation down to 10^-3 mBar. Environmental control options available for the HCS421VXY can increase the vacuum pressure limit to add high-vacuum compatibility [10^-6 mBar], or humidity control functionality via our RHC01 relative humidity controller. | |
- Rough Vacuum [10^-3 mBar] / +0.5 Bar gas-purge (Standard) PN: HCS421VXY |
- High Vacuum [10^-6 mBar] PN: HCS421HVXY+ |
- Add Humidity Control with RHC01 PN: RHC01 |
|
Vacuum Port Configuration By default, the HCS421VXY comes with 1 stainless steel vacuum bellows and one aluminum vacuum blank. For easy use with external vacuum gauges, the stage can be configured with a second vacuum bellows that can easily be removed or installed as needed. Secondary vacuum bellows are sold separately, or can be pre-installed before delivery. | |
- 1x SS vacuum bellows, 1x vacuum blank F0V (Standard) PN: HCS421VXY |
- 2x SS vacuum bellows + 1x vacuum blank F0V PN: HCS421VXY-SSKF16 |
Mounting Adapters Mounting adaptors allow the HCS421VXY to be easily mounted onto your optical instrument of choice. All HCS421VXY models include side-mounted L-brackets and tapped holes on the base for easy mounting to any instrument. Optional mounting adaptors are available for Instec's TPM-CX40, Olympus, Leica, and many more microscope models. For the full list of mounting adaptors, contact sales@instec.com. Note: Vertical mounting configurations may require sample fixture clips (SFC) to ensure compatibility. | |
- None (Standard) |
- Vertical |
- Horizontal |
- Vertical with rotation |
Special options for HCS421VXY+ customizable model
Upgrade to HCS421VXY+ for access to special options including:
Transmission Aperture Some applications require larger transmission apertures than others- whether it be to see more of a sample, allow for more light transmission, or for compatibility with specialized instruments. However, larger transmission apertures also result in larger thermal gradients across the observation area, decreasing the temperature uniformity. Tune the HCS421VXY+ transmission aperture size to optimize the temperature uniformity while maximizing the transmission aperture size. | |
- 2mm aperture (Standard) |
- 4mm aperture PN: AP4-HCS421VXY |
Electrical Feedthroughs For applications that require optical access with simultaneous temperature control and electrical testing, a range of vacuum-tight electrical feedthroughs may be added to the HCS421VXY+ customizable model. The HCS421VXY+ supports up to 2 additional standalone electrical feedthroughs, which may consist of a combination of up to 2 of any electrical feedthrough types. BNC type feedthroughs are available in two configurations: Grounded, where the outer BNC conductor is connected to earth ground for shielding, and Floating, where the outer BNC conductor is left floating relative to earth ground allowing the shielding conductor to be used as a second signal conductor. The Floating BNC configuration includes a second pogo-pin terminal for each feedthrough. | |
- 1x or 2x vacuum blanks (Standard) PN: F0V-HCS421VXY |
- 1x or 2x Grounded BNC Connectors with pogo pin terminals PN: F1V-HCS421VXY |
- 1x or 2x Floating BNC Connectors with 2x pogo pin terminals each PN: F2V-HCS421VXY |
- Customized standalone electrical feedthrough (DB-9, ribbon-cable, fiber optic, RF and much more!) |
Electrically Biased Sample Area For some applications, applying an electrical bias to the back of a sample is essential. in such cases, the HCS421VXY can be customized to include an electrically floating surface area that may be biased via an electrical feedthrough. | |
- Electrically Grounded (Standard) |
- Electrically Floating with Electrical feedthrough for applying bias PN: HCS421VXYF |
Upgraded Temperature Controller Options
The HCS421VXY is designed for use with Instec's mK2000 series temperature controllers. While the switching PID controller is recommended for most applications, in cases where low electrical noise is critical, the temperature controller may be upgraded to an LVDC output model. Furthermore, the mK2000 itself can be customized with various add-on options such as additional monitoring sensors, analog voltage output and more.... | |
- mK2000B with switching PID output (Standard) PN: mK2000B |
- mK2000B with LVDC output upgrade PN: mK2000B-LVDC |
- Additional monitoring sensor for mK2000B PN: mK2000B-TMK |
- Advanced controller upgrades (see mK2000B page for details) Ask sales@instec.com for more details |
Custom Options
If you can imagine it, Instec can make it! Instec offers complete customization services to tailor any of our standard products to your unique application. | |
- KBr, Kapton windows with custom lid |
- Custom covers |
- SMA feedthroughs |
- Floating surface area with Triaxial BNC |
- Custom sample holder |
... And much more! |
Contact sales@instec.com for more information
Temperature Range |
-190°C to 600°C |
-190°C to 400°C |
RT to 1000°C |
-40°C to 120°C |
-190°C to 400°C |
Atmospheric Control |
✓ |
✓ |
✓ |
✓ |
✓ |
Sample Area |
Ø28mm |
Ø28mm |
16 x 16mm |
42mm x 42mm |
38mm x 50mm |
Sample Cooling |
LN2 |
LN2 |
– * |
TEC |
LN2 |
Thermal Block |
Silver |
Silver |
Silicon Carbide |
Anodized Aluminum |
Anodized Aluminum |
Electrical Feedthroughs |
Up to 4 |
Up to 6 |
Up to 4 |
Up to 4 |
Up to 4 |
XY Manipulation |
✓ |
✓ |
✓ |
✓ |
Other products to consider... | |
HCP621G heating and cooling plate with Ø28mm sample area. Temperature range -190°C to 600°C. Gas tight chamber with gas purge capabilities. Includes option to add electrical feedthroughs. Has a higher max temperature, but does not have a transmission aperture, and does not support evacuation. Highly compatible with reflection-mode optical instruments. |
|
HCS601GXY-IRM FTIR heating and cooling stage with 24mm x 24mm sample area. Temperature Range -190°C to 600°C. Gas tight chamber with gas purge capabilities. CWD=10.5mm WD=10.5mm, cone angle>100°C. Includes IR windows. Has a wider temperature range, but a slightly smaller sample area, and is optimized for IR applications. |
|
HCS350G-TNS heating and cooling stage for tensile force measurements. 26mm x 16mm sample area. Temperature range -190°C to 350°C. Gas tight chamber with gas purge capabilities. Apply up to 200 N force. Specialized for experiments requiring applied force control with simultaneous optical access. |