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Thermoelectric stage with gastight chamber for inverted microscope operation
-25°C - 120°C by using thermoelectric heating and cooling
Ideally designed for samples in Petri dishes up to φ 40.5 mm or 25mm x 75mm glass microscope slides
Convenient design for long duration experiments without liquid nitrogen
TSA12Gi
PRODUCT INQUIRY -
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DESCRIPTION
Instec's TSA12Gi is Thermoelectric Heater/Cooler (Peltier) thermal stage designed specifically for inverted optical microscopes. It features a large gastight sample chamber, thermoelectric temperaature control, wide adjustable viewing window, and customizable base plate.
The TSA12Gi's sample chamber can accommodate 35mm petri dish specimens and glass slides up to 53mm x 78mm(optional). Dual thermoelectric modules heat and cool with electrical current only, eliminating the need for an expensive chiller or a supply of liquid nitrogen. TEC cooling is especially convenient compared to LN2 for performing long duration experiments at cold temperatures, as it simplifies the system set up, reduces operating costs, and requires much less maintenance.
The gastight sample chamber can be used to prevent oxidation via gas-purge, aid in humidity studies, and makes for more efficient use of expensive reaction gasses. Purge gas is pre-heated/cooled before entering the sample chamber for improved thermal performance. The removable lid windows can be defrosted for low temperature operation, and blank ports are available for adding the user's own atmospheric sensors, e.g., CO2, humidity, etc. In addition, an optional inner lid is available for better thermal uniformity. The TSA12Gi base plate is removable and replaceable, and can be customized to fit a specific microscope stage.
TSA12Gi is an ideal solution for applications such as Cell Culturing, Biology, and Thermal Microscopy for inverted microscopes.
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THERMAL FEATURES
Temperature Control
mK2000 with bidirectional LVDC output
Thermal Block
Black anodized aluminum
Sample Thermal Cover
Removable inner sample cover with additional window
Minimum Temperature
-20°C with C100W
-25°C with upgraded CW5000 with 5°C coolant
Maximum Temperature
90°C (optional upgrade for 120°C available)
Temperature Sensor
100 Ω Platinum RTD
Maximum Heating Rate
+12°C per minute at 37°C
Maximum Cooling Rate
-6°C per minute at 0°C
Minimum Heating and Cooling Rate
±0.01°C per minute
Temperature Resolution
0.01°C
Temperature Stability
±0.05°C
Power Supply
Universal power input - 150W Max (not including water cooling accessories)
Software
Windows software to record and export temperature-time data
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OPTICAL FEATURES
Optical Access
Reflection and transmission capability
Optical Windows
Removable and exchangeable windows permit full-spectrum transparency
Minimum Objective Working Distance
5.4 mm
Minimum Condenser Working Distance
22.8 mm
Top Window
Ø27mm aperture (Ø 31.75mm x 1mm glass)
Top Viewing Angle
±31° from normal
Inner lid aperture
Ø20mm aperture (Ø 25mm x 0.2mm glass)
Transmission Aperture
12 mm diameter (5mm aperture for better temperature uniformity available)
Bottom Window
Ø36mm aperture (Ø 40mm x 1mm glass)
Bottom Viewing Angle
±68° from normal
Window Defrost
Integrated top and inner window defroster
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STRUCTURAL FEATURES
Sample Area
40.5 mm diameter to fit 35 mm Petri dishes, Option for 25mm x 75mm glass slides
Enviromental Control
Sealed chamber for positive pressure gas purge up to 0.5Bar, very rough vacuum down to 1mBar
Chamber Height
12 mm with removeable inner cover
20 mm without removeable inner cover
Frame/ TEC Cooling
Integrated TEC cooling with included C100W water-cooler. Optional chiller upgrade for increased temperature range
Mounting
Base model includes tapped holes on frame. Horizontal mounting adaptors for specific instruments are available by request
Frame Dimensions
160 mm x 110 mm x 40 mm
Weight
1000 g
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OPTIONS
TSA12Gi Base-Model Options
Add-on options for the TSA12Gi base model are listed below. These items can be added at any time, and can even be installed by the end-user in the field.
TEC/ Frame Cooling Upgrades
The standard TSA12Gi comes with a C100W benchtop water circulator that enables sample cooling down to -20°C. By adding ice to the C100W reservoir, the minimum achievable temperature is -25°C, which can be maintained for a short duration until the ice melts.
To maintain these lower temperatures for longer intervals, alternative water cooling options are available. The CW5000 is a
recirculating chiller that uses a compact refrigeration system to cool and circulate water as low as 5°C, allowing the TSA12Gi to
maintain -25°C indefinetly.
Note: Note: Cooling for extended durations in ambient conditions will lead to condensation on the thermal area and sample. The TSA12Gi chamber is sealed for light positive pressure gas purge prevent condensation while cooling.
- C100W Water Circulator (Standard)
Multi-purpose benchtop water circulator
PN: C100W
- CW5000 Industrial Chiller
Upgraded water chiller with refrigeration
capabilities, capable of cooling water to 5°C.
PN: CW5000
- Vertical mounting adaptors
Mounting Adaptors
Mounting adaptors allow the TSA12GI to be easily mounted onto your optical instrument of choice. All TSA12Gi models include tapped holes on the base for easy mounting to any instrument or surface. Optional mounting adaptors are
available for specific inverted microscopes. For the full list of mounting adaptors,
contact sales@instec.com.
- L-Brackets and tapped holes (Standard)
- Horizontal
- Vertical
- Vertical with rotation
Window Material
The TSA12Gi utilizes removable and exchangable windows to optimize preformance for any wavelength. Base-model options are
shown below, but specialized options are available by request with the TSA12Gi+ model.
- Quartz Glass (Standard)
PN: WQ1-R0317-T10, WQ1-R025-T02, WQ1-R040-T10
- BaF2 Glass for IR
PN: WBaF2-R0317-T10, WBaF2-R025-T02, WBaF2-R040-T10
- Soda Lime Glass (Standard)
PN: WG1-R0317-T10, WG1-R025-T02, WG1-R040-T10
- ZnSe Glass for IR
PN: WZnSe-R0317-T10, WZnSe-R025-T02, WZnSe-R040-T10
- CaF2 Glass for IR
PN: WCaF2-R0317-T10, WCaF2-R025-T02, WCaF2-R040-T10
- Saphire Glass for UV/IR
PN: WSP-WCaF2-R0317-T10, WSP-R025-T02, WSP-R040-T10
Special options for the TSA12Gi+ customizable model
Upgrade to the TSA12Gi+ for access to special options including:
Transmission Aperture Adjustment
Some applications require larger transmission apertures than others- whether it be to see more of a sample, allow for more light transmission, or for
compatibility with specialized instruments. However, larger transmission apertures also result in larger thermal gradients across the observation area,
decreasing the temperature uniformity. Tune the TSA12Gi+ transmission aperture size to optimize the temperature uniformity while maximizing the
transmission aperture size.
- 5mm aperture (Standard)
- 2mm aperture
PN: TS102VXY-AP2
Upgraded Temperature Controller Options
The TSA12Gi is designed for use with Instec's mK2000 series temperature controllers. TEC stages like the TSA12Gi come standard with a bidirectional
output LVDC mK2000B temperature controller. The mK2000 itself can be customized with various add-on options such as additional monitoring sensors,
analog voltage output and more....
- Additional monitoring sensor for mK2000B
PN: mK2000B-TMK
- Advanced controller upgrades (see mK2000B
page for details)
Ask sales@instec.com for more details
Custom Options
If you can imagine it, Instec can make it! Instec offers complete customization services to tailor any of our standard products to your unique application.
- KBr, Kapton windows with custom lid
- Custom covers
- SMA feedthroughs
- Expanded temperature range
- Custom sample holder
... And much more!
Contact sales@instec.com for more information