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For correlative cryo-light (inverted) and cryo-electron microscopy (CLEM)
-190°C - 150°C with resistive heating and liquid nitrogen cooling option
3x3 EM sample holder supports up to nine 3mm samples for imaging via bright field and fluorescence light microscopy
Grid holder designed for vitreous operation and transfer far below the amorphous ice phase transition temperature
CLM77Ki
PRODUCT INQUIRY -
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DESCRIPTION
Instec's CLM77Ki is a specialized stage designed for use on any uinverted microscope for correlative cryo-light and cryo-electron microscopy (CLEM). The specially designed grid holder allows up to nine 3 mm EM grids to be imaged via bright field and flourescence light microscopy for determination of regions and/or cellular events of interest for later observation in the electron microscope. The grid holder is designed to keep the grids vitreous - well below the amorphous ice phase transition temperature - during tranfer of grids into the grid holder and then into the CLM77Ki stage. To eliminate frost build-up on samples, the chamber can be gas purged and the grid holder has an integrated slide cover that is closed during transfer from liquid nitrogen into the CLM77Ki chamber.
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THERMAL FEATURES
Temperature Control
mK2000 with programmable precision switching PID method
Thermal Block
Black anodized aluminum body
Sample Thermal Cover
Integrated slide cover on grid holder to eliminate moisture condensation on samples
Temperature Minimum
-190°C (with optional liquid N2 cooling)
Temperature Maximum
120°C
Temperature Sensor
100 Ω Platinum RTD
Maximum Heating Rate
+60°C per minute at 100°C
Maximum Cooling Rate
-50°C per minute at 100°C
Minimum Heating and Cooling Rate
±0.01°C per minute
Temperature Resolution
0.01°C
Temperature Stability
±0.05°C (>25°C), ±0.1°C (<25°C)
Power Supply
Universal power input
Software
Windows software to record and export temperature-time data
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OPTICAL FEATURES
Optical Access
Reflection and transmission capability
Optical Windows
Removable and exchangeable windows permit full-spectrum transparency
Minimum Objective Working Distance
5.0 mm
Minimum Condenser Working Distance
19.3 mm
Top Aperture
19 mm diameter
Top Viewing Angle
±17.61° from normal
Transmission Aperture
2 mm diameter
Bottom Aperture
46 mm diameter（9x）
Bottom Viewing Angle
±51.34° from normal
Window Defrost
Integrated external window defrost
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STRUCTURAL FEATURES
Sample Area
Holds 9 grids of 3mm in diameter
Grid Height
0.20 mm
Sample Loading
Quick sample handling with side-loading/unloading holder Frame Cooling
Integrated frame cooling with optional chiller system
Mounting
Horizontal mounting capability
Frame Dimensions
160.8 mm x 109.8 mm x 26 mm
Weight
1300 g