PRODUCTS
ELECTRICAL PROBE SYSTEMS
THERMAL PLATES
LIQUID CRYSTAL SYSTEMS
RESOURCES
SUPPORT
ABOUT
For correlative cryo-light (upright) and cryo-electron microscopy (CLEM)
-190°C - 150°C with resistive heating and liquid nitrogen cooling option
Grid holder allows nine EM grids, 3 mm round, to be imaged via bright field and fluorescence light microscopy to improve productivity
Grid holder designed for vitreous operation and transfer far below the amorphous ice phase transition temperature
Instec's CLM77K is a specialized stage designed for use on any upright microscope for correlative cryo-light and cryo-electron microscopy (CLEM). The specially designed grid holder allows up to nine 3 mm EM grids to be images via bright field and flourescence light microscopy for determination of regions and/or cellular events of interest for later observation in the electron microscope. The grid holder is designed to keep the grids vitreous - well below the amorphous ice phase transition temperature - during tranfer of grids into the grid holder and then into the CLM77K stage. To eliminate frost build-up on samples, the chamber can be gas purged and the grid holder has an integrated slide cover that is closed during transfer from liquid nitrogen into the CLM77K chamber.
Temperature Control |
mK2000 with programmable precision switching PID method |
Thermal Block |
Black anodized aluminum body |
Sample Thermal Cover |
Integrated slide cover on grid holder to eliminate moisture condensation on samples |
Temperature Minimum |
-190°C (with optional liquid N2 cooling) |
Temperature Maximum |
120°C |
Temperature Sensor |
100 Ω Platinum RTD |
Maximum Heating Rate |
+60°C per minute at 100°C |
Maximum Cooling Rate |
-50°C per minute at 100°C |
Minimum Heating and Cooling Rate |
±0.01°C per minute |
Temperature Resolution |
0.01°C |
Temperature Stability |
±0.05°C (>25°C), ±0.1°C (<25°C) |
Power Supply |
Universal power input |
Software |
Windows software to record and export temperature-time data |
Optical Access |
Reflection and transmission capability |
Optical Windows |
Removable and exchangeable windows permit full-spectrum transparency |
Minimum Objective Working Distance |
5.0 mm |
Minimum Condenser Working Distance |
17.6 mm |
Top Window Aperture |
46 mm diameter |
Top Viewing Angle |
±45.73° from normal |
Transmission Aperture |
2 mm diameter(9x) |
Bottom Window Aperture |
19 mm diameter |
Bottom Viewing Angle |
±9.95° from normal |
Window Defrost |
Integrated external window defrost |
Sample Area |
Holds 9 grids of 3mm in diameter |
Grid Height |
0.25 mm |
Sample Loading |
Quick sample handling with side loading/unloading holder |
Frame Cooling |
Integrated frame cooling with optional chiller system |
Mounting |
Horizontal mounting capability |
Frame Dimensions |
218.8 mm x 116.8 mm x 24.5 mm |
Weight |
1500 g |